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Computer-aided modeling and optimization for capsaicinoid production by immobilized Capsicum frutescens cells.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/2286/
EMT-12-93
 
Title Computer-aided modeling and optimization for capsaicinoid production by immobilized Capsicum frutescens cells.
 
Creator Suvarnalatha, G.
Chand, N.
Ravishankar, G. A.
Venkataraman, L. V.
 
Subject 31 Food Additives
30 Spices/Condiments
 
Description Production of capsaicinoids by immobilized cell cultures of Capsicum entrapped in calcium alginate matrix is influenced by several factors related to immobilization techniques, including media composition and the biosynthetic capability of cells. Central composite rotatable design (CCRD), one of the response surface methodologies, was used to arrive at optimal conditions for maximizing the yields of capsaicinoids in only 53 experiments, instead of 15,625. Immobilization of cells in 2.5% sodium alginate with 15 min contact time in 60 mM calcium chloride and culture in Murashige and Skoog medium supplemented with 3 mg l-1 2,4-dichlorophenoxyacetic acid and 0.2% curdlan gave the highest yield of 220 micrograms g-1 in 3 days.
 
Date 1993
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2286/1/Enzyme_and_Microbial_Technology%2C_Volume_15%2C_Issue_8%2C_August_1993%2C_Pages_710-715.pdf
Suvarnalatha, G. and Chand, N. and Ravishankar, G. A. and Venkataraman, L. V. (1993) Computer-aided modeling and optimization for capsaicinoid production by immobilized Capsicum frutescens cells. Enzyme and Microbial Technology, 15 (8). pp. 710-715. ISSN 0141-0229