ICAR Research Data Repository for Knowledge Management
Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum).
IR@CSIR-CFTRI
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http://ir.cftri.com/2381/
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| Title |
Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum).
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| Creator |
Ramadoss, C. S.
Shenoy, B. C. Borthakur, A. |
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| Subject |
29 Protein Chemistry
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| Description |
A haemoprotein was purified to apparent homogeneity from Bengal-gram seeds. The purified protein exhibited an absorption maximum at 412 nm (Soret band) that upon reduction with dithionite gave rise to a shift in the Soret band to 426 nm with concomitant appearance of an alpha-band at 559 nm and a beta-band at 530 nm. In the reduced state the Bengal-gram haemoprotein showed reactivity towards CO, nitrite and hydroxylamine. SDS/polyacrylamide-slab-gel electrophoresis showed that the haemoprotein has Mr 78,000. Gel-filtration and ultracentrifugal analyses suggest that the Bengal-gram haemoprotein is oligomeric in nature. Since it differs from photosynthetic membrane cytochrome b-559 in solubility in buffer, in reactivity towards CO and in molecular size, it appears to be a novel haemoprotein b-559.
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| Date |
1987
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/2381/1/Biochem.%20J.%20%281987%29%20243%2C%20723-728.pdf
Ramadoss, C. S. and Shenoy, B. C. and Borthakur, A. (1987) Isolation and characterization of a novel haemoprotein b-559 from Bengal gram (Cicer arietinum). The Biochemical journal, 243 (3). pp. 723-8. ISSN 0264-6021 |
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