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In vitro conversion of pteroylglutamic acid to citro vorum factor by rat liver enzymes.

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BBA-02-60
 
Title In vitro conversion of pteroylglutamic acid to citro vorum factor by rat liver enzymes.
 
Creator Noronha, J. M.
Sreenivasan, A.
 
Subject 16 Enzyme Chemistry
 
Description The enzymes in rat liver which convert pteroylglutamic acid to citrovorum factor
are found to reside in the mitochondria and are dissociable into two fractions, one
bringing about the initial reduction of pteroylglutamic acid to dihydropteroylglutamic
acid and the other effecting the final reduction to tetrahydropteroylglutamic
acid followed by formylation to the citrovorum factor-active derivatives; the former
is easily released into the supernatant in isotonic saline, while the latter is bound
more firmly in the mitochondrion.
The enzyme complex has been partially purified from 0.05 ~o sodium desoxycholate
mitochondrial extract by precipitation at 30-5o ~o (NH4)~SO~ saturation.
Reducing agents act as non-specific stabilizers and a nitrogen atmosphere is
better for CF synthesis when compared to ascorbic acid addition. Serine is the most
effective formyl donor. TPNH, ATP, Mg +รท, homocysteine and pyridoxal phosphate
are important, in that order, for optimal conversion. Their role in the conversion
mechanism has been discussed.
 
Date 1960
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2416/1/Biochimica_et_Biophysica_Acta%2C_Volume_44%2C_1960%2C_Pages_64-71.pdf
Noronha, J. M. and Sreenivasan, A. (1960) In vitro conversion of pteroylglutamic acid to citro vorum factor by rat liver enzymes. Biochemica Biophysica Acta, 44. pp. 64-71.