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A central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level.

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EMT-06-01
 
Title A central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level.
 
Creator Kiran, K. R.
Manohar, B.
Divakar, S.
 
Subject 04 Fermentation Technology
 
Description Porcine Pancreas lipase (PPL) was employed for the reaction between lactic acid and lauric acid at bench-scale level. A Response Surface
Methodological (RSM) study was conducted by employing a five-level, five variable, central composite rotatable design (CCRD) in order
to understand the esterification behavior of PPL in the lauroyl lactic acid synthesis. Five important variables were considered, namely,
enzyme/substrate (E/S) ratio (0.09 –1.14 Activity Units/mmole - AU/mmol), lactic acid concentration (5–25 mmol), incubation period (6–54
h), buffer volume (0–0.2 ml) and buffer pH Values (4.0–8.0). Highest ester yield of 6.8 mmol was predicted at the lowest E/S ratio of 0.09
AU/mmol. Lower E/S ratios gave higher yields and higher E/S ratios gave lesser yields. This behavior clearly explained the competitive
nature of binding between lauric and lactic acids for the same binding site on the enzyme. Addition of buffer in terms of both volume and
pH did not have a profound effect on increase in ester yield. Predicted yields showed good validation with experimental yields when
experiments corresponding to selected points on the contour plots were carried out.
 
Date 2001
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2431/1/Enzyme_and_Microbial_Technology_29_%282001%29_122-128.pdf
Kiran, K. R. and Manohar, B. and Divakar, S. (2001) A central composite rotatable design analysis of lipase catalyzed synthesis of lauroyl lactic acid at bench-scale level. Enzyme and Microbial Technology, 29 (2/3). 122-128, 13 ref..