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Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus.

IR@CSIR-CFTRI

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Title Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus.
 
Creator Aparna, H. S.
Salimath, P. V.
 
Subject 03 Proteins
27 Dairy products
 
Description Acidic glycoproteins were isolated from buffalo colostrum at
pH 4.6 and their yields were highest -in the first day
colostrum, but decreased drastically in the third to fifth day
samples. They were purified on DEAE-cellulose and CM-cellulose
columns and exhibited complex nature. The concentrations of
carbohydrates and proteins varied from 2.1-13.9 % and 80-96 % ,
respectively, on dry weight basis. The SDS-PAGE analysis
showed the heterogeneous nature of the fractions and some of
the bands also stained positive with periodate-Schiff’s
reagent indicating their glycoprotein nature. The compositional
analysis of the glycans revealed the presence of
L-fucose, D-galactose, D-mannose, N-acetyl-D-glucosamine,
N-acetyl-D-galactosamine and N-acetyl neuraminic acid in
different proportions. Some of the isolated fractions were
found to enhance the growth of Bifidobacterium bifidus var.
Pennsylvanicus significantly at low concentrations.
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2645/1/Nutr%20Res%201999%2019%20295-.pdf
Aparna, H. S. and Salimath, P. V. (1999) Acidic glycoproteins of buffalo colostrum and their influence on the growth of Bifidobacterium bifidus. Nutrition Research, 19 (2). 295-303, 29 ref..