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Alpha-D-Mannosidase from Lycopersicon esculentum II.

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Relation http://ir.cftri.com/2772/
 
Title Alpha-D-Mannosidase from Lycopersicon esculentum II.
 
Creator Suvarnalatha, G.
Prabha, T. N.
 
Subject 05 Enzymes
03 Tomato
 
Description were puri_ed by ion!exchange chromatography on DEAE sephadex A!49 followed by gel _ltration on sephadex G!099[ The two
isoforms were named isoform I and II "minor and major forms\ respectively# which di}ered in their abundance\ their elution pro_le
on both ion!exchange and gel _ltration column chromatography[ Isoforms I and II showed 5 and 13) of the total activity\
respectively[ Both the isoforms had an acid pH optimum "3[4# and were thermally stable at 54> for up to 04 min[ Isoform I showed
a broad temp optimum between 44 and 54> whereas isoform II had an optimal activity at 54>[ The Km values of isoforms I and II
for p!nitrophenyl!a!D!mannopyranoside were 0[00 and 0[94 mM\ respectively[ The activity of isoform I was inhibited by Hg1� "0
mM# whereas isoform II was inhibited by Cu1� "9[0 mM# and Hg1� "0 mM#[ Both the isoforms were free of other glycosidases[
Puri_ed isoform II had a SDS Mr of ca 38,000.
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/2772/1/Phytochemistry%2C%20Volume%2050%2C%20Issue%207%2C%2010%20April%201999%2C%20Pages%201111-1115.pdf
Suvarnalatha, G. and Prabha, T. N. (1999) Alpha-D-Mannosidase from Lycopersicon esculentum II. Phytochemistry, 50 (7). 1111-1115, 19 ref..