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Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity.

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Title Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity.
 
Creator Shivakumar, P
Krishna, Nand.
 
Subject 04 Fermentation Technology
05 Enzymes
 
Description Samples from biogas digesters, sewage ponds, animal
house effluents and food processing wastes were used in enrichment systems seeking
anaerobic bacteria producing pectinases. Among the 46 anaerobic consortia developed from
various samples, four showed high pectinase activity under static anaerobic conditions.
Investigation of fermentation variables showed the optimum conditions for pectinase activity
were pH 7*0,45"C and 72 h of growth with 0.5% pectin in the cultivation medium. A 1.4- to
1 -6-fold increase in the pectinase activity was achieved under these conditions. The maximum
yield of enzymes (62-72 U ml- ' of pectinase, 4-74 U ml- ' of polygalacturonase, 113.30 U
ml-' of pectin lyase, 2-10 U ml-' of pectinesterase, 0.75 U ml- ' of total cellulase and 9.27
U ml-' of xylanase) was recorded with the consortia C-S2 developed from decomposed plant
samples collected from a pond.
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3014/1/Letters%20in%20Applied%20Microbiology%201995.20%2C%20117-119.pdf
Shivakumar, P and Krishna, Nand. (1995) Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity. Letters in Applied Microbiology, 20 (2). 117-119, 15 ref..