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Application of central composite rotatable design to lipase catalysed synthesis of m-cresyl acetate.

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Relation http://ir.cftri.com/3063/
 
Title Application of central composite rotatable design to lipase catalysed synthesis of m-cresyl acetate.
 
Creator Manohar, B.
Divakar, S.
 
Subject 16 Enzyme Chemistry
 
Description Esterification of m-cresol with acetic acid using porcine pancreas lipase (PPL) was investigated by response surface
methodology (RSM). A central composite rotatable design (CCRD) involving 32 experiments of five variables at
five levels was employed to analyse the esterification behaviour. The effect of five variables studied namely, m-cresol concentrations (0.005–0.025 mol), enzyme/substrate ratios (0.18–1.22 activity units/millimol, AU/mmol), incubation periods (6–54 h), pH (4–8) and buffer volumes (0–0.2 ml) was useful in arriving at an optimum ester yield. The methodology projected conditions for higher yields up to 6.0 mmol. Validation experiments carried out under these
predicated conditions showed good correspondence between experimental and predicted yields. CCRD treatment clearly showed the inhibitory nature of m-cresol in the esterification process. The reaction required the presence of buffer for better conversions and a minimum amount of 0.1 ml buffer was found necessary for this reaction. Buffer
pH values around 6.0 and below appeared to favour better esterification than those at pH values in the range 6.0–
8.0. However an optimum condition for maximum yield was: incubation period: 54 h; buffer volume: 0.2 ml; pH: 8;
E/S ratio: 0.83 AU/mmol; m-cresol: 0.02 mol; predicted yield: 6.0 mmol; experimental yield: 6.4 mmol.
 
Date 2002
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3063/1/World_J_Microbiol_Biotechnol_2002_18_8_745-751.pdf
Manohar, B. and Divakar, S. (2002) Application of central composite rotatable design to lipase catalysed synthesis of m-cresyl acetate. World Journal of Microbiology and Biotechnology, 18 (8). pp. 745-751.