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Aqueous two-phase extraction in combination with ultrafiltration for downstream processing of Ipomoea peroxidase.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/3088/
JFE-32-02
 
Title Aqueous two-phase extraction in combination with ultrafiltration for downstream processing of Ipomoea peroxidase.
 
Creator Srinivas, N. D.
Barhate, R. S.
Raghavarao, K. S. M. S.
 
Subject 10 Plants
05 Processing and Engineering
 
Description The partition behaviour of a plant peroxidase extracted from the leaves of Ipomoea palmetta was studied in aqueous two-phase
systems. The influence of various parameters such as system pH, phase composition, phase polymer molecular weight and concentration
of neutral salt on differential partitioning of the enzyme and cell debris into opposite phases were evaluated. Desirable
conditions for differential partitioning were found in systems having polyethylene glycol of lower molecular weight 1550, where the
enzyme partitioned to the bottom phase and the cell debris partitioned to the top phase. Optimum conditions for extraction of the
enzyme were observed in a polyethylene glycol (PEG-1550)/KH2PO4 system having 2% NaCl. Hence, using this system, extraction,
enrichment and purification of the enzyme was performed on a larger-scale (1-l scale) in combination with ultrafiltration to obtain
about 76% recovery, 5.9-fold purity and 9.7-fold activity concentration of the enzyme.
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3088/1/Journal_of_Food_Engineering_54_%282002%29_1-6.pdf
Srinivas, N. D. and Barhate, R. S. and Raghavarao, K. S. M. S. (2002) Aqueous two-phase extraction in combination with ultrafiltration for downstream processing of Ipomoea peroxidase. Journal of Food Engineering, 54 (1). 1-6, 18 ref..