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Ascorbic acid-induced loss of a pediocin-encoding plasmid in Pediococcus acidilactici CFR K7.

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Relation http://ir.cftri.com/3113/
WJMB-03-00
 
Title Ascorbic acid-induced loss of a pediocin-encoding plasmid in Pediococcus acidilactici CFR K7.
 
Creator Ramesh, A.
Halami, P. M.
Chandrashekar, A.
 
Subject 04 Microbiology
 
Description A strain of Pediococcus acidilactici CFR K7 grown in presence of 1 mM ascorbate for 18 h resulted in 35%
colony-forming units (CFU) irreversibly losing their ability to produce pediocin. Agarose gel electrophoresis of
plasmid DNA and dot-blot hybridization showed concomitant loss of a 7.8 kb plasmid coding for pediocin from
these colonies. Sensitivity of these colonies to nitrofurantoin was also observed after ascorbate treatment. Since
ascorbic acid is a readily available and non-hazardous compound in contrast to other conventional plasmid curing
agents, the possibility of its use in determining plasmid-encoded traits in food grade lactic acid bacteria is also
proposed.
 
Date 2000
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3113/1/World_Journal_of_Microbiology_and_Biotechnology_2000_16_695-.pdf
Ramesh, A. and Halami, P. M. and Chandrashekar, A. (2000) Ascorbic acid-induced loss of a pediocin-encoding plasmid in Pediococcus acidilactici CFR K7. World Journal of Microbiology and Biotechnology, 16 (7). 695-697, 14 ref..