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Binding of Mg(II) by the 11S fraction of soybean proteins.

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Title Binding of Mg(II) by the 11S fraction of soybean proteins.
 
Creator Rao, A. G. A.
Rao, M. S. N.
 
Subject 03 Proteins
05 Soya bean
 
Description Binding of Mg(II) by the 11s fraction of soybean
proteins has been measured in 0.1 M borate buffer
of pH 7.8 by equilibrium dialysis. The binding
sites are possibly the imidazole groups of the histidine
residues of the protein molecule. NaCl at
0.5 M concentration suppresses binding. Addition
of utea or .prior treatment of the protein with
EDTA reduces binding, EDTA treatment causes
dissociation of the proteiD which is partly re-versed by the addition of Mg(II). No confonnational
change occurs in the protein due to the
binding of Mg(II). The protein, both before and
after EDTA treatment, is quantitatively precipitated
by 1 x 10-2 M Mg(II). NaCI at 0.5 M concentration
suppresses precipitation. Addition of
Mg(II) to tbe water extract of defatted soybean
yields a precipitate which consists almost entirely
of the 11S fraction.
 
Date 1975
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/3156/1/Journal%20of%20Agricultural%20and%20Food%20Chemistry%2C%20Volume-23%284%20%281975%29%20657-661.pdf
Rao, A. G. A. and Rao, M. S. N. (1975) Binding of Mg(II) by the 11S fraction of soybean proteins. Journal of Agricultural and Food Chemistry, 23 (4). 657-661, 16 ref..