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Binding of Zn(II) by the 11S fraction of soybean proteins.

IR@CSIR-CFTRI

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Title Binding of Zn(II) by the 11S fraction of soybean proteins.
 
Creator Rao, A. G. A.
Rao, M. S. N.
 
Subject 03 Proteins
05 Soya bean
 
Description Binding of Zn(II) by the l1S fraction of soybean proteins in 0.2 M acetate buffer of pH 6.5 was determined
by equilibrium dialysis. The Scatchard plot of binding data indicated heterogeneity of binding sites.
Binding occurs possibly at the imidazole groups of histidine residues. Addition of 0.5 M NaCI to the
buffer or prior treatment of the protein with EDTA had no effect on binding. Zn(1I) binding did not
change the sedimentation velocity pattern of the protein or its s20w value. It did not affect toe fluorescence
spectrum and ORD in the visible range. However, it increased heat coagulation of the protein. At 4
X 10-3M and higher Zn(II) concentrations the protein was quantitatively precipitated. Precipitation
at low concentrations of Zn(II) was suppressed by NaCI but was not at higher concentrations.
 
Date 1976
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3157/1/Journal%20of%20Agricultural%20and%20Food%20Chemistry%2C%20Volume-24%283%20%281976%29%20487-490.pdf
Rao, A. G. A. and Rao, M. S. N. (1976) Binding of Zn(II) by the 11S fraction of soybean proteins. Journal of Agricultural and Food Chemistry, 24 (3). 487-490, 21 ref..