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Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase.

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Relation http://ir.cftri.com/3191/
 
Title Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase.
 
Creator Jegan Roy, J.
Abraham, T. E.
Abhijith, K. S.
Sujith Kumar, P. V.
Thakur, M. S.
 
Subject 07 Food Engineering
11 Food Biochemistry
 
Description Cross-linked enzyme crystals (CLECs) are a versatile form of biocatalyst that can also be used for biosensor application. Laccase from Trametes versicolor (E.C.1.10.3.2) was crystallized, cross-linked and lyophilized with �-cyclodextrin. The CLEC laccase was found to be highly active towards phenols like 2-amino phenol, guaiacol, catechol, pyrogallol, catechin and ABTS (non-phenolic). The CLEC laccase was embedded in 30% polyvinylpropylidone (PVP) gel and mounted into an electrode to make the sensor. The biosensor was used to detect the phenols in 50–1000 �mol concentration level. Phenols with lower molecular weight such as 2-amino phenol, catechol and pyrogallol gave a short response time where as the higher molecular weight substrates like catechin and ABTS had comparatively a long response time. The optimum pH of the analyte was 5.5–6.0 when catechol was used as substrate. The CLEC laccase retained good activity for over 3 months.
 
Date 2005
 
Type Article
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3191/1/biosenbioelect2005-21%281%29206-211.pdf
Jegan Roy, J. and Abraham, T. E. and Abhijith, K. S. and Sujith Kumar, P. V. and Thakur, M. S. (2005) Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase. Biosensors and Bioelectronics, 21 (1). pp. 206-211.