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Characterization of cell wall components from the endosperm of sorghum varieties varying in hardness.

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CP-05-93
 
Title Characterization of cell wall components from the endosperm of sorghum varieties varying in hardness.
 
Creator Kavitha, R.
Chandrashekar, A.
 
Subject 06 Sorghum
28 Polysaccharide Chemistry
 
Description Cell walls, isolated from 2 var. of sorghum [M-35-1 (soft) and E-35-1 (hard)] endosperm varying in hardness, were extracted with 1, 4 or 8M alkali and subsequently neutralized to give an insoluble 'A' and a soluble 'B' fraction. The soluble 'B' fractions had higher arabinose-to-xylose and pentose-to-hexose ratios compared with their respective 'A' fractions. The residue after extraction with 8M alkali in the soft var. contained 16% pentoses, whereas the residue from the hard var. had only traces of pentoses. On further fractionation of 1M 'B' on DEAE cellulose, a glucose-rich unbound fraction and a bound fraction containing a considerable amount of pentoses were obtained. Prolamins (sorghum storage proteins) were associated with isolated cell wall fractions. Antibodies were raised against 3 of the cell wall polymer fractions and were capable of recognizing specific and common epitopes. Results indicate that the more loosely held polymers are rich in glucose, while the tightly held polymers, probably attached to cellulose, are made up of pentoses.
 
Date 1993
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3374/1/Carbohydrate_Polymers%2C_Volume_22%2C_Issue_2%2C_1993%2C_Pages_107-115.pdf
Kavitha, R. and Chandrashekar, A. (1993) Characterization of cell wall components from the endosperm of sorghum varieties varying in hardness. Carbohydrate Polymers, 22 (2). 107-115, 22 ref..