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Chickpea seed proteins: modification during germination.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/3447/
Phyto-09-78
 
Title Chickpea seed proteins: modification during germination.
 
Creator Ganesh, Kumar
Venkataraman, L. V.
 
Subject 29 Protein Chemistry
22 Legumes-Pulses
 
Description Changes in the proteins of chickpea during a 12-day germination period are reported using techniques of gel filtration, DEAE-cellulose chromatography, polyacrylamide gel (PAG) electrophoresis and ultracentrifugation. In the ultracentrifuge, the total proteins of dormant seeds resolve into 3 components which have the sedimentation coeff. of 2.2 S, 6.9 S and 10.3 S resp. On germination, the presence of fractions of lower sedimentation coeff. indicates possible degradation of these components; in the early stages, the degradation rate of the 7 S fraction is higher, while the 10 S fraction is broken down faster in the later stages. Gel filtration experiments indicate the possibility of degradation of high polymer into intermediary products. Increase in the relative mobility of protein components on PAG and elution constant on DEAE-cellulose chromatographs indicates an increase in the net negative charge of the protein fractions. The accumulation of subunits of the proteins is negligible during the germination period.
 
Date 1978
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3447/1/Phytochemistry%2C_Volume_17%2C_Issue_4%2C_1978%2C_Pages_605-609.pdf
Ganesh, Kumar and Venkataraman, L. V. (1978) Chickpea seed proteins: modification during germination. Phytochemistry, 17 (4). 605-609, 30 ref..