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Chitosanolysis by a pectinase isozyme of Aspergillus niger - a non-specific activity

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Title Chitosanolysis by a pectinase isozyme of Aspergillus niger - a non-specific activity
 
Creator Kittur, F. S.
Vishu Kumar, A. B.
Lalitha, R. Gowda
Tharanathan, R. N.
 
Subject 05 Enzymes
 
Description An isozyme of Aspergillus niger pectinase could depolymerize chitosan and its derivatives. The isozyme showed optimum
depolymerization at pH 3.0 and 47 8C and exhibited classical Michaelis–Menten kinetics with a Km and a Vmax of 3.12 mg ml21 and
153.85 nmol min21 mg21, respectively, towards chitosan, which were higher than those for chitosan derivatives. The low molecular weight
chitosans (LMWC) obtained were in the range of 6–20 kDa depending on the reaction conditions. Identification of glucosamine (GlcN) and
N-acetylglucosamine (GlcNAc) oligomers as depolymerized products indicated that the isozyme cleaved both GlcN-GlcN and GlcNAc-
GlcN linkages. This study suggests the possible use of pectinase isozyme in place of chitosanase, which is expensive and unavailable in bulk
quantity for the production of LMWC and chitooligomers
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3449/1/Carbohydrate_Polymers%2C_Volume_53%2C_Issue_2%2C_1_August_2003%2C_Pages_191-196.pdf
Kittur, F. S. and Vishu Kumar, A. B. and Lalitha, R. Gowda and Tharanathan, R. N. (2003) Chitosanolysis by a pectinase isozyme of Aspergillus niger - a non-specific activity. Carbohydrate Polymers, 53 (2). pp. 191-196.