ICAR Research Data Repository for Knowledge Management
Colorimetric determination of sinalbin (sinapin p-hydroxybenzylglucosinolate).
IR@CSIR-CFTRI
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http://ir.cftri.com/3515/
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| Title |
Colorimetric determination of sinalbin (sinapin p-hydroxybenzylglucosinolate).
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| Creator |
Raghavan, B.
Shankaranarayana, M. L. Nagalakshmi, S. Natarajan, C. P. |
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| Subject |
01 Analysis
04 Mustard seed |
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| Description |
The determination of sinalbin in white mustard seed via the product of enzymic hydrolysis, p-hydroxybenzyl isothiocynate (p-HBI), is described. Reaction of p-HBI with NaOH produces p-hydroxybenzyl alcohol and NaCNS, which is converted into CNBr and treated with pyridine-benzidine reagent. Resulting colour is linear at 518 nm between 0.1 and 0.5 mug CNS/ml. 1 mole sinalbin produces 1 mole CNS-- with a recovery of 90%.
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| Date |
1972
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://ir.cftri.com/3515/1/Mikrochimica%20Acta%2C%20Volume-6%28%20%281972%29%20818-822.pdf
Raghavan, B. and Shankaranarayana, M. L. and Nagalakshmi, S. and Natarajan, C. P. (1972) Colorimetric determination of sinalbin (sinapin p-hydroxybenzylglucosinolate). Mikrochimica Acta, 6. pp. 818-822. |
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