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Dissociation and denaturation behaviour of sesame-alpha-globulin in sodium dodecyl sulphate solution.

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Title Dissociation and denaturation behaviour of sesame-alpha-globulin in sodium dodecyl sulphate solution.
 
Creator Prakash, V.
Nandi, P. K.
 
Subject 03 Proteins
01 Oilseeds
 
Description The effect ofanionic detergent, sodium dodecyl sulphate, on the majorprotein, alpha-globulin
ofsesame seed(Sesamum indicum L.) has been investigated by geljiltration, sedimentation
re!ocity, vi,rcosity, optical rotation, difference spectra and jluoresceuce measurements.
The detergem causes dissociation of the protein first and then denaturation.
In the deTergent concentration range of 1.75-4.0 x 10 - ~ M foul' components are
observed in the ultracentrifuge. The specific rotation ofthe protein increases with the
detergent concentration above 2.5 x 10 M and shows a cooperative transition
between 3-8 x 10 M detergent suggesting conformational change; above 8 x 10 M
detergent The value of - [alpha] does not change. The reduced viscosity nred however,
increases above 2.5 x 10- 3 M detergent and does not attain a plateau value. The
difference spectrum ofthe protein indicates that both tryptophan and tyrosine groups
have been affected by the detergent. The fluorescence intensity decreases and the
maxima shifts towards red in the detergent solution resulting in an 'isoemissive point'
at 355 nm. The double difference spectra in sucrose-detergent protein system show that
below 5ยท0 x 10- 3 M detergent, the difference absorption andjluorescence spectrum result
from the binding of the detergent near the chromophoric groups and are not due to
conformational change. Binding studies by equilibrium dialysis indicate the presence
of ~ 50 binding sites in the protein and a binding constant of 3.0 x 103
 
Date 1976
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/3798/1/International%20Journal%20of%20Peptide%20and%20Protein%20Research%2C%20Volume-8%28%20%281976%29%20385-392.pdf
Prakash, V. and Nandi, P. K. (1976) Dissociation and denaturation behaviour of sesame-alpha-globulin in sodium dodecyl sulphate solution. International Journal of Peptide and Protein Research, 8. pp. 385-392.