ICAR Research Data Repository for Knowledge Management
Enzymic synthesis of N-acetyl-L-phenylalanine in Escherichia coli K12.
IR@CSIR-CFTRI
View Archive Info| Field | Value | |
| Relation |
http://ir.cftri.com/4124/
|
|
| Title |
Enzymic synthesis of N-acetyl-L-phenylalanine in Escherichia coli K12.
|
|
| Creator |
Krishna, R. V.
Krishnaswamy, P. R. Rajagopal Rao, D. |
|
| Subject |
16 Enzyme Chemistry
|
|
| Description |
Cell-free extracts of Escherichia coli K12 catalyse the synthesis of N-acetyl- L-phenylalanine from acetyl-CoA and L-phenylalanine. 2. The acetyl-CoA-Lphenylalanine a-N-acetyltransferase was purified 160-fold from cell-free extracts. 3. The enzyme has a pH optimum of 8 and catalyses the acetylation of L-phenylalanine. Other L-amino acids such as histidine and alanine are acetylated at slower rates. 4. A transacylase was also purified from E. coli extracts and its substrate specificity studied. 5. The properties of both these enzymes were compared with those of other known amino acid acetyltransferases and transacylases. |
|
| Date |
1971
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Language |
en
|
|
| Rights |
—
|
|
| Identifier |
http://ir.cftri.com/4124/1/Biochem.%20J.%20%281971%29%20124%2C%20905-913.pdf
Krishna, R. V. and Krishnaswamy, P. R. and Rajagopal Rao, D. (1971) Enzymic synthesis of N-acetyl-L-phenylalanine in Escherichia coli K12. Biochemical Journal, 124. pp. 905-913. |
|