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Estimation of vitamin B12 (hydroxocobalamin) in proteolysed liver extracts.

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Title Estimation of vitamin B12 (hydroxocobalamin) in proteolysed liver extracts.
 
Creator Sreenivasamurthy, V.
Swaminathan, M.
Subrahmanyan, V.
 
Subject 04 Vitamins
 
Description The stability of hydroxocobalamin to ascorbic
acid-sodium ascorbate (AAM) in the
presence of varying concentrations of iron
has been studied. Iron In concentrations
higher than 3-5 ['g./ml. prevents the destruction
of hydroxocobalamin by AAM to varying,
extents, 100 per cent protection being, attained
at 32 l~g,. level. At levels less than 1ยท5 ['g.,
iron does not exhibit any protective action.
Cyanocobalam.in is not destroyed under the
above conditions. Hydroxocobalamin present
in proteolysed liver extracts couId be selectively
destroyed by treatment with AAM if
the iron content of the extract is adjusted to
about 1 ['g./ml. by suitable dilution of the extract.
Based on the above findings, a method
for the determination of hydroxocobalamin
content of liver extracts has been developed.
The results of analysis of a few samples of
proteolysed liver extracts indicate that about
50-60 per cent of the vitamin B,. activity of the
extracts is due to hydroxocobalarnill.
 
Date 1956
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/4142/1/Journal%20of%20Scientific%20and%20Industrial%20Research%2C%20Volume-15C%28%20%281956%29%20215-217.pdf
Sreenivasamurthy, V. and Swaminathan, M. and Subrahmanyan, V. (1956) Estimation of vitamin B12 (hydroxocobalamin) in proteolysed liver extracts. Journal of Scientific and Industrial Research, 15C. pp. 215-217.