Record Details

Influence of cultural factors on the production of chitosanase by Rhodotorula gracilis CFR-1.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/4412/
 
Title Influence of cultural factors on the production of chitosanase by Rhodotorula gracilis CFR-1.
 
Creator Somashekar, D.
Richard, Joseph.
 
Subject 04 Microbiology
 
Description Rhodotorula gracilis (ATCC-90950) was found to secrete an extracellular chitosan hydrolysing enzyme constitutively, but
was incapable of growth on chitosan as carbon source. The enzyme production appeared to be growth associated in all the
three media (Minimal salts + peptone, Yeast extract-peptone-dextrose and potato dextrose) tested. In the potato dextrose
broth a peak level of enzyme was found 30 h of culture, after which there was apparent inactivation of enzyme. Such a
pattern of enzyme loss was not noticed in the other two media. The organism could grow on glucose, sucrose, xylose,
succinate, glycerol or mannitol, each employed as sole carbon source and produced chitosanase with these substrates.
Enzyme was produced in ammonium sulphate, ammonium chloride, sodium nitrate, urea as nitrogen source, but not in
ammonium nitrate. Several amino acids also supported chitosanase production.
 
Date 1996
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/4412/1/Indian_Journal_of_Microbiology_196_36_213-216.pdf
Somashekar, D. and Richard, Joseph. (1996) Influence of cultural factors on the production of chitosanase by Rhodotorula gracilis CFR-1. Indian Journal of Microbiology, 36 (4). pp. 213-216.