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Interaction of allylisothiocyanate with bovine serum albumin.

IR@CSIR-CFTRI

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Title Interaction of allylisothiocyanate with bovine serum albumin.
 
Creator Kishore Kumar Murthy, N. V.
Narasinga Rao, M. S.
 
Subject 12 Allergy & Toxicology
 
Description The interaction of aiJYli~()thiocyanate with bovine serum albumin
was monitored by fluorescence titration. The interaction was weak with an apparent
asso.::iation constau[ of 2 x 1O~. The interaction was unaffected in the pH range
of 5·0 to 8·3 and by Nae!. However, the addition of dioxane upto 4% increased
the value of the assodation constant. N-Methyl bovine serum albumin and bOvine
serum albumin with sulphydryl groups blocked had the same affinitY for allylisothiocyanate
suggesting that amino and sulphydryl groups may not be involved
in the interaction. Polyacrylamide gel electrophoresis and estimation of available
lysine suggested that there were perhaps two types of groups involved in the interaction
of allylisothiocyanate with bovine serum albumin.
 
Date 1979
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/4490/1/Journal%20of%20Biosciences%2C%20Volume-1%282%20%281979%29%20125-133.pdf
Kishore Kumar Murthy, N. V. and Narasinga Rao, M. S. (1979) Interaction of allylisothiocyanate with bovine serum albumin. Journal of Biosciences, 1 (2). pp. 125-133.