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Intracellular distribution and biosynthesis of ubiquinone in rat liver in carbon tetrachloride liver injury.

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Title Intracellular distribution and biosynthesis of ubiquinone in rat liver in carbon tetrachloride liver injury.
 
Creator Aiyar, A. S.
Sreenivasan, A.
 
Subject 04 Biosynthesis
09 Human Physiology
 
Description The fundamental metabolic deviation by which
carbon tetrachloride administration results in
fatty degeneration of liver is not yet clear. In recent
reports on the changes in composition and function
of the fatty liver cell particular attention has been
paid to the mitochondria. A damage to mitochondrial
integrity is accompanied by major biochemical
alterations (Dianzani, 1954, 1955, 1956;
Dianzani & Viti, 1955; Kasbekar, Lavate, Rego &
Sreenivasan, 1959). Inhibition of certain enzymes
(Dianzani, 1953) and activation of others (Lehn.-
inger, 1951; Berthet & de Duve, 1951; Schneider
& Hogeboom, 1952a, b; Hogeboom & Schneider,
1953) latent in fresh mitochondria, especially of
adenosine triphosphatase (Kielley & Kielley, 1951;
SREENIVASAN
Potter & Recknagel, 1951; Copenhaver & Lardy,
1952; Lardy & Wellman, 1952, 1953) have been
reported.
Dianzani & Viti (1955) have observed decreases
in both cytochrome c and pyridine nucleotides in
fatty livers. Work in this Laboratory (Nadkarni,
Wagle & Sreenivasan, 1957) has further shown that
the biosynthesis in vivo of pyridine nucleotides
from intraperitoneally administered nicotinamide
is less in the carbon tetrachloride-treated animals.
A decrease in hepatic coenzyme A has also been
reported in fatty degeneration (Severi & Fonnesu,
1956; Heim, Leuschner & Ott, 1956). In view of
the changes in cytochrome c and pyridine nucleotides,
and in coenzyme A, which is known to be necessary for the biosynthesis of ubiquinone (cf.
A iyar, Sulebele, Rege & Sreenivasan, 1959; Aiyar &
Sreenivasan, 1961), a study of the alterations in
ubiquinone levels in fatty livers is of interest.
In this paper we present data on the intracellular
distribution of ubiquinone and on its
biosynthesis in vitro by liver slices from labelled
precursors. Ubiquinone in the liver slices was isolated by chromatography
(Festenstein at al. 1955), and the counts were read
in a Tracerlab SC-16 windowless gas-flow counter in conjunction
with a Tracerlab SC-51 autoscaler. The ubiquinone
was later determined spectrophotometrically
(Crane et al. 1959).
Nitrogen was estimated by a micro-Kjeldahl method
(Umbreit, Burris & Stauffer, 1946).
 
Date 1962
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/4501/1/Biochemical_Journal_1962_82_179-182.pdf
Aiyar, A. S. and Sreenivasan, A. (1962) Intracellular distribution and biosynthesis of ubiquinone in rat liver in carbon tetrachloride liver injury. Biochemical Journal, 82. pp. 179-182.