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Isolation, purification and some properties of hemicellulase from Fusarium

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Relation http://ir.cftri.com/4525/
CR-01-81
 
Title Isolation, purification and some properties of hemicellulase from Fusarium
 
Creator Wankhede, D. B.
Vijayalakshmi, K. R.
Raghavendra Rao, M. R.
 
Subject 05 Enzymes
04 Microbiology
 
Description An extracellular hemicellulase from a soil fungus (Fusarium sp.) grown on a
medium containing groundnut hemicellulose B was purified 76-foId by ammonium
sulphate fractionation, chromatography on DEAE-cellulose, and gel filtration on
Sephadex G-100. It was found to be homogeneous by disc-gel electrophoresis at
pH 8. It showed optimal activity at pH 5.6 and 37”. It was observed that groundnut
and sesame hemicellulose B were degraded considerably (m 80 and 58 %, respectively)
by the purified hemicellulase, whereas glucomannan and xylan from groundnut
were comparatively poorly hydrolysed (N 30-40 %)_
 
Date 1981
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/4525/1/Carbohydrate_Research%2C_Volume_96%2C_Issue_2%2C_16_October_1981%2C_Pages_249-257.pdf
Wankhede, D. B. and Vijayalakshmi, K. R. and Raghavendra Rao, M. R. (1981) Isolation, purification and some properties of hemicellulase from Fusarium. Carbohydrate Research, 96. pp. 249-257.