Isolation, purification and some properties of hemicellulase from Fusarium
IR@CSIR-CFTRI
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Relation |
http://ir.cftri.com/4525/
CR-01-81 |
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Title |
Isolation, purification and some properties of hemicellulase from Fusarium
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Creator |
Wankhede, D. B.
Vijayalakshmi, K. R. Raghavendra Rao, M. R. |
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Subject |
05 Enzymes
04 Microbiology |
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Description |
An extracellular hemicellulase from a soil fungus (Fusarium sp.) grown on a medium containing groundnut hemicellulose B was purified 76-foId by ammonium sulphate fractionation, chromatography on DEAE-cellulose, and gel filtration on Sephadex G-100. It was found to be homogeneous by disc-gel electrophoresis at pH 8. It showed optimal activity at pH 5.6 and 37”. It was observed that groundnut and sesame hemicellulose B were degraded considerably (m 80 and 58 %, respectively) by the purified hemicellulase, whereas glucomannan and xylan from groundnut were comparatively poorly hydrolysed (N 30-40 %)_ |
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Date |
1981
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://ir.cftri.com/4525/1/Carbohydrate_Research%2C_Volume_96%2C_Issue_2%2C_16_October_1981%2C_Pages_249-257.pdf
Wankhede, D. B. and Vijayalakshmi, K. R. and Raghavendra Rao, M. R. (1981) Isolation, purification and some properties of hemicellulase from Fusarium. Carbohydrate Research, 96. pp. 249-257. |
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