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L-galactosidase from germinating guar (Cyamopsis tetragonolobus) seeds.

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Title L-galactosidase from germinating guar (Cyamopsis tetragonolobus) seeds.
 
Creator Shivanna, B. D.
Ramakrishna, M.
 
Subject 07 Enzyme Biochemistry
22 Legumes-Pulses
 
Description The changes in alpha galactosidase activity in guar (Cyamopsis tetragonolobus) seeds
was followed during seven days of germination. The enzyme activity was maximal on the first
day of germination and gradually decreased during subsequent days. On the second day of
germination the partially purified enzyme upon ion-exchange chromatography on CMSephadex
C-50 was resolved into a-galactosidase-A (anionic). alpha galactosidase-C1 (cationic)
and a-galactosidase-C. (cationic) and their relative proportions were 28, 12 and 60~~~,
respectively. The combined alpha galactosidase C1 and C1 activities increased in the first two days
of germination followed by significant decrease after the 3rd day onwards, whereas alpha galactosidase
·A remained fairly constant throughout the germination period, alpha Galactosidase-
A and C2 had different Km and Vmax values with p-nitrophenyl alpha galactopyranoside,
raffinose and melibiose as substrates and also differed in their thermal stabilities.
 
Date 1985
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/4536/1/Journal%20of%20Biosciences%2C%20Volume-9%281-2%20%281985%29%20109-116.pdf
Shivanna, B. D. and Ramakrishna, M. (1985) L-galactosidase from germinating guar (Cyamopsis tetragonolobus) seeds. Journal of Biosciences, 9 (1-2). pp. 109-116.