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Microscopic observation of Sudan black B staining to monitor lipid production by microbes.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/4639/
 
Title Microscopic observation of Sudan black B staining to monitor lipid production by microbes.
 
Creator Thakur, M. S.
Prapulla, S. G.
Karanth, N. G.
 
Subject 22 Lipid Chemistry
04 Microbiology
04 Fermentation Technology
 
Description In experimental investigations on the improvement of yields and
productivities of lipids from oleaginous micro-organisms in fermenters, a
bottleneck is the speedy determination of the extent of accumulation of the
intracellular lipids. The use of Sudan Black B for staining and direct
observation under the microscope enables the rapid observation of the
qualitative status of lipid production in the cells. A change in the colour of the
stain @om dark to light blue observed during the course of the fermentation
has been found to be related to a change in the extent of unsaturation of the
lipids.
 
Date 1988
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/4639/1/J%20.%20Chem.%20Tech.%20Biotechnol.%201988%2C%2042%2C%20129-134.pdf
Thakur, M. S. and Prapulla, S. G. and Karanth, N. G. (1988) Microscopic observation of Sudan black B staining to monitor lipid production by microbes. Journal of Chemical Technology and Biotechnology, 42 (2). pp. 129-134.