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Phase Sensitive Estimation Of Fluorescence Lifetime For Fiber Optic Biosensors

Electronic Theses of Indian Institute of Science

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Title Phase Sensitive Estimation Of Fluorescence Lifetime For Fiber Optic Biosensors
 
Creator Vadde, Venkatesh
 
Subject Applied Optics
Fluorescence
Fiber Optic Devices - Fluorescence
Fluorescence Spectroscopy
Phase Sensitive Fluorescence Biosensors
Fiber Optic Biosensors
Phase Sensitive Signal Processing
Fluorescence Based Oxygen Sensor
Fluorescence - Detection
Phase Sensitive Fluorescence Sensors
Phase Sensitive Signal Processing
 
Description Fluorescence lifetime determination and allied studies find application in spectroscopy in general and fiber optic biosensors in particular. Instruments and sensors cited in literature however use open loop, intensity based techniques with sophisticated detectors and components. We propose phase sensitive signal processing schemes to estimate the fluorescence lifetime using simple detectors and components, without compromising on accuracy. The performance of the schemes proposed is analysed and contrasted from a communications (signals and systems) point of view.
The resolution and sensitivity limits imposed in processing the signal, by systematic errors and additive noise, are derived for the schemes suggested. It is found that systematic errors impose a phase resolution limit of about 2°. We then study the suitability of different detectors and channels for application in phase sensitive fluorescence biosensors we analyse the effect of systematic limitations as well as additive noise, in the detection/transmission process, from the point of view of the components used. Certain fundamental limits of operation in terms of excitation intensities are derived for different detector-channel combinations, with a view to obtain a given resolution. A photodiode used with a fiber bundle is found to be sufficient for accurate phase read outs with 10"4 radians resolution. A PMT used in conjunction with a multimode fiber serves as a very good device for microsensing applications
Lastly, the biosensor for oxygen sensing, the ruthenium complex, is studied for standardisation of the sensor. We examine the quenching of fluorescence, the repeatability and reusability of the sensor, the stability of the instrument and such.
 
Publisher Indian Institute of Science
 
Contributor Vivek, Srinivas
 
Date 2005-10-04T08:51:07Z
2005-10-04T08:51:07Z
2005-10-04T08:51:07Z
1995-06
 
Type Electronic Thesis and Dissertation
 
Format 2981919 bytes
application/pdf
 
Identifier http://hdl.handle.net/2005/150
null
 
Language en
 
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