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Titania Nanotubes For Biotechnological Applications

Electronic Theses of Indian Institute of Science

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Field Value
 
Title Titania Nanotubes For Biotechnological Applications
 
Creator Murria, Priya
 
Subject Titanium Nanotubes
Bionanotechnology
Biomedical Engineering
Myoglobin Encapsulation
Titania Nanotubes - Biomedical Applications
Fe-Doped Titania Nanotubes
Fe-doped TiO2 Nanotubes
Drug Delivery
Titania Nanotube (TNT)
Nanostructured Materials
TiO2 Nanoparticles
Nanotechnology
 
Description Over the past few decades, inorganic nanostructured materials have elicited a lot of interest due to their high surface-to-volume ratio and many size dependent properties which stem from their nanoscale dimensions. Owing to these distinct properties, they have found applications in widespread fields like catalysis, energy storage, electronics, and biotechnology.
In the field of biotechnology, nanotubes and mesoporous materials are attractive vehicles for drug delivery because of their hollow and porous structures and facile surface functionalization. Their inner void can take up large amounts of drug as well as act as gates for the controlled release of drug. These hollow structures can also be used for confining biomolecules like proteins and peptides. The study on protein conformation in biocompatible materials is very important in materials sciences for the development of new and efficient biomaterials(sensors, drug delivery systems or planted devices).
Titania(TiO2)has been widely explored for applications in photovoltaic cells, batteries, desalination, sensing, and photocatalysis, to name only a few. The work presented in this thesis focuses on titania based nanostructures for drug delivery and protein confinement.
First part of the work focusses on synthesis and characterization of Fe-doped TiO2 nanotubes. Fe-doped TiO2 nanotubes were demonstrated as controlled drug delivery agents. In vitro cytotoxic effects of Fe-doped titania nanotubes were assessed by MTT assay by exposing Hela cell line to the nanotubes.
Second part of the work focusses on synthesis and characterization of TiO2 nanotubes by two synthesis procedures, namely hydrothermal and sol-gel template synthesis. Myoglobin, a model globin protein was encapsulated in hydrothermally synthesized TiO 2 nanotubes(diameter 5 nm) and sol-gel template synthesized TiO2 nanotubes(diameter 200 nm). Effect of encapsulating myoglobin these nanotubes was studied. The electrochemical activity and structure of myoglobin were studied by cyclic voltammetry and circular dichroism respectively. Direct electron transfer was found to be enhanced upon confinement in 200 nm diameter nanotubes. No such enhancement was observed upon encapsulation in hydrothermally synthesized nanotubes. In addition to this, the thermal stability of myoglobin was found to be enhanced upon confinement inside 200 nm diameter TiO 2 nanotubes.
 
Contributor Bhattacharyya, Aninda J
 
Date 2014-08-04T10:01:46Z
2014-08-04T10:01:46Z
2014-08-04
2011-07
 
Type Thesis
 
Identifier http://etd.iisc.ernet.in/handle/2005/2354
http://etd.ncsi.iisc.ernet.in/abstracts/3027/G24776-Abs.pdf
 
Language en_US
 
Relation G24776