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Electrochemical Detection Of Proteins : Myoglobin As A Case Study

Electronic Theses of Indian Institute of Science

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Field Value
 
Title Electrochemical Detection Of Proteins : Myoglobin As A Case Study
 
Creator Narayan, Karthik K
 
Subject Electrochemical Sensors
Proteins - Electrochemical Detection
Myoglobins - Electrochemical Detection
Myoglobin
Proteins as Biomarkers
Titania Nanotubes (TNT)
Heme Proteins
Myb
Biochemistry
 
Description An effective electrochemical sensor for myoglobin (Myb) detection was developed using a simple procedure of denaturing the protein with guanidine hydrochloride and detecting the released heme group by cyclic voltammetry. The concentration of denaturant was optimized to obtain maximum current response for the analyte (Myb). To improve the sensitivity of the sensor, the working electrode, glassy carbon electrode was modified with a layer of Titania nanotubes (TNT). The direct electrochemical behavior of the modified electrode (TNT-GCE) was studied using cyclic voltammetry (CV). The performance of the sensor was investigated and optimized and the system was evaluated by monitoring the Myb concentration. Despite the reduced current response for the modified electrode compared to bare GCE, the sensitivity of the system was improved significantly by overcoming the large background current due to denaturant. The developed TNT modified electrode improved the detection limit of Myb and showed good stability, sensitivity and reproducibility. Under optimal conditions, the catalytic currents are linearly proportional to the concentrations of Myb in the wide range from 50 nM to 6 M. This approach provides improved sensitivity in the given range, and may provide a novel and efficient platform for the fabrication of sensors for other heme proteins.
 
Contributor Bhattacharyya, Aninda Jiban
 
Date 2015-09-08T06:17:04Z
2015-09-08T06:17:04Z
2015-09-08
2012-11
 
Type Thesis
 
Identifier http://etd.iisc.ernet.in/handle/2005/2482
http://etd.ncsi.iisc.ernet.in/abstracts/3204/G25414-Abs.pdf
 
Language en_US
 
Relation G25414