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Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology.

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Relation http://ir.cftri.com/11477/
http://dx.doi.org/10.1021/jf500207x
 
Title Isoflavone Augmentation in Soybean Cell Cultures Is Optimized
Using Response Surface Methodology.
 
Creator Akitha Devi, M. K.
Giridhar, P.
 
Subject 04 Plant Physiology
 
Description Glycine max contains potential therapeutic isoflavones, and its productivity in plants is considerably influenced
worldwide by several biotic and abiotic factors. Optimization of soybean cell suspension cultures (Indian variety, JS 335) to
maximize the cell growth and isoflavone production in the present study was performed using response surface methodology
(RSM) with three independent variables of plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthalene acetic
acid (α-NAA), and kinetin (Kn). The maximum biomass achieved was 70.62 g/L dry weight (dw) using the optimized medium
of 2.10 mg/L 2,4-D, 5.52 mg/L α-NAA, and 0.35 mg/L Kn supplemented in the Murashige and Skoog (MS) basal medium. The
total isoflavone content of 38.59 mg/g of dw was obtained in the medium with optimized conditions of 1.33 mg/L 2,4-D, 1.76
mg/L α-NAA, and 0.15 mg/L Kn. In comparison to field-grown soybean seeds, the cell suspension cultures profoundly
augmented isoflavone concentrations. The optimized conditions for both biomass and total isoflavone content were evaluated by
superimposing the contour plots. The results suggested that the optimized medium of cell suspension cultures possibly be used
for scale-up studies in bioreactors to offer a high content of bioactive isoflavones.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11477/1/J.Agric.Food%20Chem_2014_62_3143.pdf
Akitha Devi, M. K. and Giridhar, P. (2014) Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology. Journal of Agricultural and Food Chemistry, 62. pp. 3143-3149. ISSN 0021-8561