Record Details

An OSMAC (One Strain Many Compound) Approach For the Isolation of Novel Enzyme Inhibitors.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/11510/
 
Title An OSMAC (One Strain Many Compound) Approach For the Isolation of Novel Enzyme Inhibitors.
 
Creator Prajwal, G. Waychal
 
Subject 04 Fungi
04 Fermentation Technology
 
Description Many years of study have revealed that fungi are excellent sources of novel bioactive secondary metabolites. Some of these secondary metabolites possess therapeutic qualities that improve the quality of life for millions of people. Such metabolites include well known classes such as the penicillins, cephalosporins, and statins, yet many fungi remain underexplored as sources of biologically active metabolites. The research described in this thesis employs an OSMAC (One Strain Many Compound) approach for the isolation of novel inhibitors.
The research involves investigation and isolation of bioactive compounds from the selected fungi i.e. CFR-107 which shows inhibition against various enzyme. The selected fungal strain was subjected to fermentation on fungal broth and crude extract were screened for their ability to produce novel inhibitors against various enzyme. Analysis and separation of selected fungal crude extract was done using High Performance Liquid Chromatography (HPLC) method and each separated compounds were checked for inhibition against these enzymes. In the present study, we focus on the constituents isolated from selected fungal strain having inhibitory potency along with IC50 values.
 
Contributor Avinash, P. Sattur
 
Date 2013
 
Type Student Project Report
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11510/1/prajwal%20waychal.pdf
Prajwal, G. Waychal (2013) An OSMAC (One Strain Many Compound) Approach For the Isolation of Novel Enzyme Inhibitors. [Student Project Report] (Submitted)