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Mixed reverse micelles facilitated downstream processing of lipase involving water-oil-water (WOW) liquid emulsion membrane.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/11529/
 
Title Mixed reverse micelles
facilitated downstream processing of lipase involving water-oil-water (WOW) liquid
emulsion membrane.
 
Creator Saibal, Bhowal
 
Subject 07 Food Engineering
 
Description BACKGROUND:
Mixed reverse micelles consisting of cationic and non-ionic surfactants were shown to
enhance the recovery of lipase (EC 3.1.1.3, from Aspergillus niger) in water-oil-water
(WOW) liquid emulsion membrane compared to micelles consisting of only cationic
surfactant. The mechanism of enhanced extraction was elucidated and the effect of
various operating parameters on the extraction process has been presented.
RESULTS: The extraction of lipase was found to depend on the many factors such as
concentration of cationic (CTAB) and nonionic (Tween 80) surfactants, stirring speed,
contact time, internal aqueous phase pH, feed pH, KCl concentration (internal aqueous
phase), NaCl concentration (external phase) and ratio of membrane emulsion to feed
volume. The optimum conditions for maximum lipase recovery and purification fold
(100% and 17.08 fold) were CTAB concentration 0.075 M, Tween 80 concentration
0.012 M, stirring speed 500 rpm, contact time 15 min, internal aqueous phase pH 7, feed
pH 9, KCl concentration 1 M, NaCl concentration 0.1 M and ratio of membrane emulsion
to feed volume 1:1.
CONCLUSION: Incorporation of the nonionic surfactant (Tween 80) remarkably
enhanced the activity recovery and purification fold of lipase. The optimized conditions
can be used for the primary purification of lipase. Further, it may be possible to club this
technique with other methods to meet the demand of highly purified lipase.
 
Contributor Rastogi, N. K.
 
Date 2013
 
Type Student Project Report
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11529/1/Saibal.pdf
Saibal, Bhowal (2013) Mixed reverse micelles facilitated downstream processing of lipase involving water-oil-water (WOW) liquid emulsion membrane. [Student Project Report] (Submitted)