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Reaction Kinetics of the Invertase from Yeast (S. Cerevisiae).

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Relation http://ir.cftri.com/11576/
http://doi.org/10.1007/s11483-014-9330-y
 
Title Reaction Kinetics of the Invertase from Yeast (S. Cerevisiae).
 
Creator Srinivas, Sistla
Purnima Kaul, Tiku
 
Subject 19 Yeast
 
Description Invertase converts sucrose to glucose and fructose.
The reaction mechanism for the formation of glucose and
fructose was studied by stopped flow spectrophotometer and
circular dichroism. The reaction mechanism follows biphasic
mode with rate constants of k10.0053 s−1±0.001 s−1 and k2
0.030 s−1±0.01 s−1 for 25 mM concentration of sucrose. Far
UV circular dichroic spectrum of invertase in presence of
sucrose shows 18 % increase in β conformation as a function
of time. Taken together, the invertase hydrolysis follows biphasic
mode where it undergoes conformational changes
followed by hydrolysis of the sucrose.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11576/1/Food%20Biophys_2014_9_2_179.pdf
Srinivas, Sistla and Purnima Kaul, Tiku (2014) Reaction Kinetics of the Invertase from Yeast (S. Cerevisiae). Food Biophysics, 9 (2). pp. 179-183.