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Biodegradation of Shrimp Biowaste by Marine Exiguobacterium sp. CFR26M and Concomitant Production of Extracellular Protease and Antioxidant Materials: Production and Process Optimization by Response Surface Methodology.

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Relation http://ir.cftri.com/11579/
http://doi.org/10.1007/s10126-013-9531-2
 
Title Biodegradation of Shrimp Biowaste by Marine Exiguobacterium sp.
CFR26M and Concomitant Production of Extracellular Protease
and Antioxidant Materials: Production and Process Optimization
by Response Surface Methodology.
 
Creator Anil Kumar, P. K.
Suresh, P. V.
 
Subject 07 Waste utilization
Fish
 
Description Twelve marine bacterial cultures were screened for
extracellular protease activity, and the bacterium CFR26M
which exhibited the highest activity on caseinate agar plate
was identified as an Exiguobacterium sp. Significant amount
of extracellular protease (5.9±0.3 U/ml) and antioxidant materials,
measured as 2,2′-diphenyl picrylhydrazyl (DPPH) radical
scavenging activity (44.4±0.5 %), was produced by
CFR26M in submerged fermentation using a shrimp biowaste
medium. Response surface methodology (RSM) was
employed to optimize the process variables for maximum
production of protease and antioxidant materials by
CFR26M. Among the seven variables screened by two-level
2**(7–2) fractional factorial design, the concentration of
shrimp biowaste, sugar, and phosphate was found to be significant
(p ≤0.05). The optimum levels of these variables were
determined by employing the central composite design (CCD)
of RSM. The coefficient of determination (R2) values of
0.9039 and 0.8924 for protease and antioxidant, respectively,
indicates the accuracy of the CCD models. The optimum
levels of shrimp biowaste, sugar, and phosphate were 21.2,
10.5, and 2.3 % (w/v) for production of protease and 28.8, 12,
and 0.32 % (w/v) for production of antioxidant material,
respectively. The concentration of shrimp biowaste, sugar,
and phosphate had linear and quadratic effect on both protease
and antioxidant productions. RSM optimization yielded 6.3-
fold increases in protease activity and 1.6-fold in antioxidant
material production. The crude protease of CFR26M had a
maximum activity at 32±2 °C with pH 7.6. This is the first
report on the use of marine Exiguobacterium sp. for concomitant
production of protease and antioxidant materials from
shrimp biowaste.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11579/1/Marine%20Biotech_2014_16_2_202.pdf
Anil Kumar, P. K. and Suresh, P. V. (2014) Biodegradation of Shrimp Biowaste by Marine Exiguobacterium sp. CFR26M and Concomitant Production of Extracellular Protease and Antioxidant Materials: Production and Process Optimization by Response Surface Methodology. Marine Biotechnology, 16 (2). pp. 202-218.