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Use of Extracts Cluster Analysis (Exclan) to Rapidly Screen for Enzyme Inhibitors In Microbial Fermented Extracts.

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Relation http://ir.cftri.com/11621/
 
Title Use of Extracts Cluster Analysis (Exclan) to Rapidly Screen for Enzyme Inhibitors In Microbial Fermented Extracts.
 
Creator Vasantha, K. Y.
Murugesh, C. S.
Sattur, A. P.
 
Subject 04 Fermentation Technology
 
Description The success of a new molecule discovery programme lies in the effectiveness of the primary screen for desired
activities. A rapid screen, Extracts Cluster Analysis (ExCLAN) was used for the screening of lipoxygenase inhibitors from
microbial extracts. About 50 crude extracts were randomly grouped as 5 clusters and screened for inhibition. Cluster C3,
which showed 93% inhibition, was further deconvoluted, to pinpoint isolate P25 as the extract with the inhibitor. Further
isolation and purification identified the inhibitor as sclerotiorin with an IC50 value of 4.6μM against soybean
lipoxygenase. This method helped in significantly reducing the time and number of assays with subsequent savings in
costs of conducting the assays.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11621/1/International%20Journal%20of%20Bioassays.pdf
Vasantha, K. Y. and Murugesh, C. S. and Sattur, A. P. (2014) Use of Extracts Cluster Analysis (Exclan) to Rapidly Screen for Enzyme Inhibitors In Microbial Fermented Extracts. International Journal of Bioassays. pp. 1990-1993.