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Oral Supplements of Aqueous Extract of Tomato Seeds Alleviate Motor Abnormality, Oxidative Impairments and Neurotoxicity Induced by Rotenone in Mice: Relevance to Parkinson’s Disease.

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Title Oral Supplements of Aqueous Extract of Tomato Seeds Alleviate
Motor Abnormality, Oxidative Impairments and Neurotoxicity
Induced by Rotenone in Mice: Relevance to Parkinson’s Disease.
 
Creator Krishna, Gokul
Dr., Muralidhara
 
Subject 17 Toxicology
03 Tomato
 
Description Although tomato seeds (an industrial by-product)
are known to contain several bioactive compounds,
studies describing their health effects are limited. Previously,
we evidenced that aqueous extract of tomato seeds
(TSE) markedly attenuated rotenone (ROT)-induced oxidative
stress and neurotoxicity in Drosophila system. This
study investigated the neuroprotective effect of TSE in a
chronic ROT model of neurotoxicity in mice. Initially, we
assessed the potential of oral supplements of TSE to
modulate the levels of endogenous markers of oxidative
stress in brain regions of mice. Subsequently, employing a
co-exposure paradigm, the propensity of TSE (100 mg/kg
bw, 3 weeks) to attenuate ROT-induced behavioral phenotype
(gait abnormalities, anxiety-like state), oxidative
dysfunctions and neurotoxicity was examined. We found
that mice provided with TSE supplements exhibited progressive
improvement in gait pattern and exploratory
behavior. TSE markedly offset ROT-induced oxidative
impairments, restored reduced glutathione levels, antioxidant
defenses (superoxide dismutase, glutathione peroxidase)
and protein carbonyls content in brain regions.
Specifically, TSE effectively diminished ROT induced
elevation in the activity levels of acetylcholinesterase and
restored the dopamine levels in striatum. Interestingly, in
mitochondria, TSE was able to restore the activity of
mitochondrial complexes and redox state. Collectively, our
findings in the chronic ROT model demonstrate the ability
of TSE to alleviate behavioral phenotype, oxidative stress,
mitochondrial dysfunction and neurotoxicity. Further
studies in dopaminergic cell models are necessary to
understand the precise molecular mechanism/s by which
tomato seed bioactives offer significant neuroprotection.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11625/1/Neurochemical%20Res_39_7_2014_1382.pdf
Krishna, Gokul and Dr., Muralidhara (2014) Oral Supplements of Aqueous Extract of Tomato Seeds Alleviate Motor Abnormality, Oxidative Impairments and Neurotoxicity Induced by Rotenone in Mice: Relevance to Parkinson’s Disease. Neurochemical Research, 39:. pp. 1382-1394.