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Aspergillus flavus infection and aflatoxin contamination in sorghum seeds and their biological management.

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Relation http://ir.cftri.com/11647/
http://dx.doi.org/10.1080/03235408.2013.869892
 
Title Aspergillus flavus infection and aflatoxin contamination in sorghum
seeds and their biological management.
 
Creator Divakara Shetty, T.
Mohammed, Aiyaz
Hariprasad, P.
 
Subject 04 Microbiology
06 Sorghum
 
Description In order to establish the current scenario of aflatoxigenic fungal infection and
aflatoxin contamination in sorghum seeds across India, 58 seed samples were
collected from different agro-climatic regions. Among these, 67.2% samples were
infected with Aspergillus spp. and 28% were found contaminated with aflatoxins
ranging from 0.0 to 130 μg kg−1. Greenhouse studies revealed no correlation between
incidence of Aspergillus flavus and aflatoxin content, and its effect on seed quality
parameters. Among the 37 A. flavus strains isolated, six were non-aflatoxigenic when
analysed through cultural, TLC and ic-ELISA. Seed treatment with biocontrol agents
(antagonistic Rhizobacteria and Trichoderma) suppressed the growth of A. flavus
under laboratory and significantly enhanced seed quality variables under greenhouse
conditions to a various extent. Field trials with selected biocontrol agents showed
that talcum powder formulations of Pseudomonas putida Has-1/c, Bacillus spp. 3/a,
Trichoderma asperellum M5 and T. asperellum T2 improved seedling emergence,
% nutrient accumulation in plants, increased plant biomass and 1000 seed weight.
Seeds harvested from treated plants showed significant increase in seed quality variables
under laboratory and greenhouse conditions in comparison with control, but
there was no significant difference in A. flavus infection and aflatoxin was completely
absent in all treatments.
 
Publisher Taylor & Francis
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11647/1/Archives%20Of%20Phytopathology%20And%20Plant%20ProtectionVolume%2047%2C%20Issue%2017%2C%20October%202014%2C%20pages%202141-2156.pdf
Divakara Shetty, T. and Mohammed, Aiyaz and Hariprasad, P. (2014) Aspergillus flavus infection and aflatoxin contamination in sorghum seeds and their biological management. Archives of Phytopathology and Plant Protection, 47 (17). pp. 2141-2156.