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Evaluating the effect and effectiveness of different constructs with a conserved sequence for silencing of Coffea canephora N-methyltransferases.

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Relation http://ir.cftri.com/11649/
http://dx.doi.org/10.1007/s13562-013-0224-8
 
Title Evaluating the effect and effectiveness of different constructs
with a conserved sequence for silencing of Coffea
canephora N-methyltransferases.
 
Creator Shibin, Mohanan
Satyanarayana, K. V.
Sridevi, V.
Kalpashree, Gowda
 
Subject 01 Biotechnology and Bioengineering
04 Coffee
 
Description Caffeine biosynthesis involves sequential methylation
of the purine ring by the transfer of methyl group from the
methyl donor S-adenosyl methionine. Genes that code for the
individual methyl transferase have a high degree of sequence
homology. To achieve decaffeination by genetic engineering of
coffee, the effect and efficiency of different post-transcriptional
gene silencing (PTGS) constructs were evaluated. Sense, antisense
and invert repeat constructs were developed using a
339 bp fragment from the conserved region of the coffee Nmethyltransferase
genes involved in caffeine biosynthesis.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11649/1/Journal%20of%20Plant%20Biochemistry%20and%20Biotechnology%20October%202014%2C%20Volume%2023%2C%20Issue%204%2C%20pp%20399-409.pdf
Shibin, Mohanan and Satyanarayana, K. V. and Sridevi, V. and Kalpashree, Gowda (2014) Evaluating the effect and effectiveness of different constructs with a conserved sequence for silencing of Coffea canephora N-methyltransferases. Journal of Plant Biochemistry and Biotechnology, 23 (4). pp. 399-409.