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Methylcobalamin – A form of vitamin B12 identified and characterised in Chlorella vulgaris.

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Relation http://ir.cftri.com/11664/
http://dx.doi.org/10.1016/j.foodchem.2014.08.035
 
Title Methylcobalamin – A form of vitamin B12 identified and characterised
in Chlorella vulgaris.
 
Creator Kumudha, A.
Selvakumar, S.
Dilshad, P.
Gopal, V.
Thakur, M. S.
Sarada, R.
 
Subject 01 Algae
04 Vitamins
 
Description Vitamin B12 is among the most essential biomolecules required for crucial metabolic processes in
humans. Vitamin B12 was extracted from Chlorella vulgaris biomass under aqueous conditions, partially
purified by passing the extract through amberlite XAD-2, Sep-Pak columns, and further purified by HPLC.
The target peak eluent was subjected to characterisation by tandem mass spectrometry (MS/MS),
selected ion recording (SIR) and multiple reaction monitoring (MRM) and identified as methylcobalamin
(Me-Cbl). Quantification of Me-Cbl was carried out by microbiological and chemiluminescence methods,
and found to be 29.87 ± 2 lg/100 g and 26.84 ± 2 lg/100 g dry weight, respectively. The presence of
Me-Cbl was further substantiated using gold nanoparticle (AuNPs) based aptamer analysis, and found
to be 28.02 ± 2 lg/100 g dry weight. Good similarity was observed among all the methods. Methylcobalamin,
a form of vitamin B12 was identified in C. vulgaris and this finding enhances its use as a nutritional
supplement.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11664/1/Food%20Chemistry%2C%20Volume%20170%2C%201%20March%202015%2C%20Pages%20316-320.pdf
Kumudha, A. and Selvakumar, S. and Dilshad, P. and Gopal, V. and Thakur, M. S. and Sarada, R. (2015) Methylcobalamin – A form of vitamin B12 identified and characterised in Chlorella vulgaris. Food Chemistry, 170. pp. 316-320.