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Structural analyses and immunomodulatory properties offructo-oligosaccharides from onion (Allium cepa).

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Relation http://ir.cftri.com/11665/
http://dx.doi.org/10.1016/j.carbpol.2014.09.039
 
Title Structural analyses and immunomodulatory properties offructo-oligosaccharides from onion (Allium cepa).
 
Creator Prasanna Kumar, V.
Harish Prashanth, K. V.
Venkatesh, Y. P.
 
Subject 23 Vegetables
 
Description tOnion (Allium cepa) is an immune-boosting food rich in fructans. The major aim of this study is tocharacterize and investigate the immunomodulatory properties of onion fructo-oligosaccharides (FOS).FOS was isolated from onion bulbs by hot 80% ethanol extraction (yield: ∼4.5 g/100 g fw) followedby gel permeation chromatography. NMR of onion FOS revealed unusual �-d-Glc terminal residue atthe non-reducing end. TLC and ESI–MS analyses showed that onion FOS ranged from trisaccharidesto hexasaccharides. Onion FOS (50 �g/mL) significantly increased (∼3-fold) the proliferation of mousesplenocytes/thymocytes vs. control. Further, onion FOS enhanced (∼2.5-fold) the production of nitricoxide by peritoneal exudates cells (PECs) from Wistar rats; intracellular free radicals production andphagocytic activity of isolated murine PECs were also augmented. Our structural and in vitro resultsindicate that onion FOS comprising of tri- to hexasaccharide units belongs to inulin-type fructans, andpossess immunostimulatory activities towards murine lymphocytes and macrophages.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11665/1/Carbohydrate%20Polymers%2C%20Volume%20117%2C%206%20March%202015%2C%20Pages%20115-122.pdf
Prasanna Kumar, V. and Harish Prashanth, K. V. and Venkatesh, Y. P. (2015) Structural analyses and immunomodulatory properties offructo-oligosaccharides from onion (Allium cepa). Carbohydrate Polymers, 117. pp. 115-122.