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Studies on gold nanoparticles for application in detection of aflatoxins in selected food commodities.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/11750/
 
Title Studies on gold nanoparticles for application in detection of
aflatoxins in selected food commodities.
 
Creator Abhijith, K. S.
 
Subject 01 Biotechnology and Bioengineering
10 Food Microorganisms
 
Description Nanobiotechnological approach for the detection of toxins and
pathogens for food safety has become an important field of research in recent
years. Considering the vast potentials of nanoparticles as nano labels in
biosensing, gold nanoparticle based immunosensing methodology
development for mycotoxins was attempted keeping aflatoxin B1 as model
mycotoxin. Gold nanoparticles were synthesized by different methods having
diverse opto-physical properties. Antibodies against aflatoxin B1 was raised in
avian system and used as biorecognition element. Antibodies generated
showed high affinity towards aflatoxins (2.8x107 L/M) as confirmed by
competitive ELISA. The anti aflatoxin IgY antibodies were bioconjugated with
the synthesized gold nanoparticles. Further metal enhanced luminescence
concept was studied in detail for biosensing application. Three different
luminescence concepts viz. metal enhanced fluorescence (MEF), metal
enhanced bioluminescence (MEB) and metal enhanced chemiluminescence
(MEC) were studied. The enhancement of luminescence due to the presence
of gold nanoparticles was two to ten folds with different methods. With the
basic knowledge of metal enhanced luminescence concept, three different
strategies for aflatoxin B1 detection were attempted. With MEF method,
aflatoxin B1 detection limit was found to be 5 pg/mL whereas in enhanced
chemiluminescence (EnCL) method it was 0.1 ng/g. Both the methods
showed sensitivity limit well below the EU regulations for aflatoxins (2 ng/g).
Further a field applicable semi quantitative dipstick method was developed for
rapid screening of aflatoxins. Visual detection range for immunodipstick was
found to be 1-200 ng. Further simulated real sample analysis was carried out
using food samples like wheat flour and peanut butter. Both MEF and EnCL
method showed intra and inter assay precision having RSD below 15%. The
results clearly indicated the possibility of developing robust, field applicable
immunosensor based on gold nanoparticles antibody bioconjugates for the
detection of mycotoxins like aflatoxin.
 
Contributor Thakur, M. S.
 
Date 2013-12
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11750/1/Abhijith%20Thesis.pdf
Abhijith, K. S. (2013) Studies on gold nanoparticles for application in detection of aflatoxins in selected food commodities. PhD thesis, University of Mysore.