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Kinetic modeling, production and characterization of an acidic lipase produced by Enterococcus durans NCIM5427 from fish waste.

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Relation http://ir.cftri.com/11770/
http://dx.doi.org/10.1007/s13197-013-1141-5
 
Title Kinetic modeling, production and characterization of an acidic
lipase produced by Enterococcus durans NCIM5427 from fish
waste.
 
Creator Vrinda, R.
Louella Concepta, Goveas
Prakash, M. Halami
Bhaskar, N.
 
Subject 05 Enzymes
07 Waste utilization
Fish
 
Description Enterococcus durans NCIM5427 (ED-27), capable
of producing an intracellular acid stable lipase, was isolated
from fish processing waste. Its growth and subsequent lipase
production was optimized by Box Behneken design (optimized
conditions: 5 % v/v fish waste oil (FWO), 0.10 mg/ml
fish waste protein hydrolysates (FWPH) at 48 h of fermentation
time). Under optimized conditions, ED-27 showed a 3.0
fold increase (207.6 U/ml to 612.53 U/ml) in lipase production,
as compared to un-optimized conditions. Cell growth and
lipase production wasmodeled using Logistic and Luedeking-
Piretmodel, respectively; and lipase production by ED-27 was
found to be growth-associated. Lipase produced by ED-27
showed stability at low pH ranges from 2 to 5 with its optimal
activity at 30 °C , pH4.6; showedmetal ion dependent activity
wherein its catalytic activity was activated by barium, sodium,
lithium and potassium (10 mM); reduced by calcium and
magnesium (10 mM). However, iron and mercury (5 mM)
completely inactivated the enzyme. In addition, modifying
agents like SDS, DTT, β-ME (1%v/v) increased activity of
lipase of ED-27; while, PMSF, DEPC and ascorbic acid
resulted in a marked decrease. ED-27 had maximum cell
growth of 9.90309 log CFU/ml under optimized conditions
as compared to 13 log CFU/ml in MRS. The lipase produced
has potential application in poultry and slaughterhouse waste
management.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11770/1/Journal%20of%20Food%20Sci%20%26%20Tech_2015_52_3_1328.pdf
Vrinda, R. and Louella Concepta, Goveas and Prakash, M. Halami and Bhaskar, N. (2015) Kinetic modeling, production and characterization of an acidic lipase produced by Enterococcus durans NCIM5427 from fish waste. Journal of Food Science and Technology, 52 (3). pp. 1328-1338.