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Gold nanoparticles enhanced chemiluminescence – a novel approach for sensitive determination of aflatoxin-B1.

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Relation http://ir.cftri.com/11772/
http://dx.doi.org/10.1039/c3ay40694f
 
Title Gold nanoparticles enhanced chemiluminescence – a
novel approach for sensitive determination of
aflatoxin-B1.
 
Creator Abhijith, K. S.
Ragavan, K. V.
Thakur, M. S.
 
Subject 01 Biotechnology and Bioengineering
 
Description The present paper describes the effect of gold nanoparticles on the enhancement of chemiluminescence,
widely known as metal enhanced chemiluminescence. Here for the first time we have studied the effect of
gold nanoparticles on two enhanced chemiluminescence systems, alkaline phosphatase–disodium
2-chloro-5-(4-methoxyspiro{1,2-dioxetane-3,20-(50-chloro)tricyclo[3.3.1.13,7]decan}-4-yl)-1-phenylphosphate
and luminol–urea hydrogen peroxide-horseradish peroxidase . In both the systems, up to 100%
enhancement of chemiluminescence was observed in the presence of citrate capped gold nanoparticles.
On the other hand cetyl trimethyl ammonium bromide capped gold nanorods did not exhibit much
effect on chemiluminescence. Based on the results, a dipstick based enhanced chemiluminescence based
method was developed for the sensitive detection of aflatoxin-B1 in water samples. The minimum
detectable limit for aflatoxin-B1 was 0.1 ng mL�1 with a linearity in the range of 0.5–20 ng (R2 ¼ 0.994).
The results were well correlated with those obtained by HPLC analysis (linearity 3–200 ng, R2 ¼ 0.99).
This enhanced chemiluminescence based immunodipstick method can be used for the development of a
quantitative test kit for the determination of aflatoxins.
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11772/1/Analy%20Methods%202012_4_4250.pdf
Abhijith, K. S. and Ragavan, K. V. and Thakur, M. S. (2013) Gold nanoparticles enhanced chemiluminescence – a novel approach for sensitive determination of aflatoxin-B1. Analytical Methods, 5. pp. 4838-4845.