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Application of green synthesis of gold nanoparticles for sensitive detection of aflatoxin B1 based on metal enhanced fluorescence.

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Relation http://ir.cftri.com/11773/
http://dx.doi.org/10.1039/c2ay25979f
 
Title Application of green synthesis of gold nanoparticles for sensitive detection of
aflatoxin B1 based on metal enhanced fluorescence.
 
Creator Abhijith, K. S.
Thakur, M. S.
 
Subject 10 Plants
01 Biotechnology and Bioengineering
 
Description A green synthesis approach is reported for the synthesis of gold nanoparticles (GNPs) using the leaf
extract of Coreopsis lanceolata. The synthesized GNPs were effectively utilized in the detection of
aflatoxins at low concentrations based on the metal enhanced fluorescence principle. The structural
characteristics of the GNPs were analyzed by UV-Vis spectroscopy, TEM and spectrofluorimetry.
Bioreduction of auric chloride by C. lanceolata leaf extract resulted in the synthesis of spherical,
homogenous GNPs. Further GNPs were conjugated with anti-aflatoxin antibodies and aflatoxin B1 to
study the metal enhanced charge transfer between the nanoparticles and aflatoxins, which can be
effectively utilized in the detection of aflatoxins at very low concentrations. The studies showed up to a
10-fold increase in the fluorescence intensity of aflatoxin B1 at picogram levels. Linearity was found to
be in the range of 10 picogram to 500 picogram with a regression coefficient R2 ΒΌ 0.970. The limit of
detection was found to be 5 picogram. Thus the fluorescent GNPs synthesized by this green route can
be effectively used in the detection of aflatoxins at sub-nanogram levels by utilizing the metal enhanced
fluorescence principle.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11773/1/Analy%20Methods%202012_4_4250.pdf
Abhijith, K. S. and Thakur, M. S. (2012) Application of green synthesis of gold nanoparticles for sensitive detection of aflatoxin B1 based on metal enhanced fluorescence. Analytical Methods, 4. pp. 4250-4256.