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Assessment of Acrylamide Degradation Potential of Pseudomonas aeruginosa BAC-6 Isolated from Industrial Effluent.

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Relation http://ir.cftri.com/11779/
http://dx.doi.org/10.1007/s12010-014-0923-1
 
Title Assessment of Acrylamide Degradation Potential
of Pseudomonas aeruginosa BAC-6 Isolated
from Industrial Effluent.
 
Creator Vijayashree, Chandrashekar
Chandrika, Chandrashekar
Rajath, Shivakumar
Sourav, Bhattacharya
Arijit, Das
Bhaskar, Gouda
 
Subject 02 Bacteriology
17 Toxicology
 
Description Acrylamide finds diverse industrial applications but is considered an environmental
threat because of its neurotoxic, carcinogenic, and teratogenic effects. Certain bacteria enzymatically
degrade acrylamide to acrylic acid and ammonia. The present investigationwas carried out to isolate
and identify an acrylamide-degrading bacterium from industrial effluent. Bacterial growth and extent
of acrylamide degradation in the presence of different acrylamide concentrations, nutrients, varied
range of pH, and temperature were analyzed.Among the eight acrylamide-degrading isolates, isolate
BAC-6 demonstrated the highest degradation, and based upon the partial 16S rDNA sequencing, it
was identified as Pseudomonas aeruginosa. P. aeruginosa BAC-6 grew over a wide range of
acrylamide concentrations, but the highest degradationwas recorded at 500 mg/L concentrationwith
concomitant cell growth. Among the carbon supplements, mannitol supported the highest growth
and degradation. Maximum degradation was reported at neutral pH. A mesophilic temperature
range (25–40 °C) facilitated conducive bacterial growth followed by degradation. The highest
degradation and bacterial growth were observed at 30 and 35 °C, respectively. Thus, it could be inferred from the present investigation that cultural conditions strongly affected the degradation
potential of P. aeruginosa BAC-6 and advocated the utilization of the isolate in bioremediation of
sites polluted with acrylamide.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11779/1/Applied%20Biochemistry%20and%20Biotechnology%20July%202014%2C%20Volume%20173%2C%20Issue%205%2C%20pp%201135-1144.pdf
Vijayashree, Chandrashekar and Chandrika, Chandrashekar and Rajath, Shivakumar and Sourav, Bhattacharya and Arijit, Das and Bhaskar, Gouda (2014) Assessment of Acrylamide Degradation Potential of Pseudomonas aeruginosa BAC-6 Isolated from Industrial Effluent. Applied Biochemistry and Biotechnology, 173 (5). pp. 1135-1144.