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Permeabilized probiotic Lactobacillus plantarum as a source of b-galactosidase for the synthesis of prebiotic galactooligosaccharides.

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Relation http://ir.cftri.com/11781/
http://dx.doi.org/10.1007/s10529-013-1345-9
 
Title Permeabilized probiotic Lactobacillus plantarum as a source
of b-galactosidase for the synthesis of prebiotic
galactooligosaccharides.
 
Creator Gobinath, D.
Prapulla, S. G.
 
Subject 04 Microbiology
 
Description Permeabilized probiotic Lactobacillus plantarum
was used as a source of b-galactosidase for the
synthesis of galactooligosaccharides (GOS) from lactose.
b-galactosidase activity was highest when galactose
(1,724 Miller Units) was used as a carbon source
compared to lactose, sucrose or glucose at 37 �C, 18 h.
Permeabilized cells had the highest transgalactosylation
activity resulting in 34 % (w/w) GOS synthesis from
40 % (w/v) lactose at 50 �C over 12 h. HPLC revealed
that the GOS were composed of 13 % disaccharides
(non-lactose), 17 % trisaccharides and 4 % tetrasaccharides
that were further confirmed by ESI–MS.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11781/1/Biotechnology%20Letters%20January%202014%2C%20Volume%2036%2C%20Issue%201%2C%20pp%20153-157.pdf
Gobinath, D. and Prapulla, S. G. (2014) Permeabilized probiotic Lactobacillus plantarum as a source of b-galactosidase for the synthesis of prebiotic galactooligosaccharides. Biotechnology Letters, 36 (1). pp. 153-157.