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The 18S rDNA gene discriminates between red-listed and unexplored ethnomedicinal species of Myristicaceae restricted to humid tropics of India.

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Relation http://ir.cftri.com/11791/
http://dx.doi.org/10.1007/s10722-013-0060-7
 
Title The 18S rDNA gene discriminates between red-listed
and unexplored ethnomedicinal species of Myristicaceae
restricted to humid tropics of India.
 
Creator Sheeja, T. E.
Rosana, O. B.
Swetha, V. P.
Shalini, R. S.
Siju, S.
Dhanya, R.
 
Subject 04 Medicinal Plants
 
Description A potentially diagnostic 18S rDNA (ribosomal
DNA) gene was amplified reliably from red-listed
ethnomedicinal species of Myristica and its wild and
related genera. Individuals from nine species of Myristicaceae
were utilized for the study. The sequences
ranged from 1,767 to 1,794 nucleotide (nt) in length. The
GC content (%) varied from 52.77 to 51.04. The
frequencies (%) of nt were A (23.31), T (23.82), C
(24.48) and G (28.39). The alignment of all sequences
produced 195/1,516 variable sites and 1,257/1,516
conserved sites. Total numbers of single nucleotide
polymorphism (SNP) sites found in the alignment were
146/1,516. Knema andamanica (Warb.) W.J. de Wilde
was the most distinct that included 18 variable regions
and 15 InDel with 27 SNP sites, specific to this species.
The identified regions fromnine species ofMyristica and
its wild and closely related genera were deposited in the
GenBank Database (Accession numbers JN228257-
JN228265). Comparison of morphological identifications
and phylogenetic analysis indicated that the specimens
were correctly assigned on the basis of a short
stretch of 18S rDNA (*1,600 bp) making this a
potentially useful marker for the rapid molecular assignment
of an unknown related species also. Significant
sequence homology ranging from 72 to 99 % was
observed on comparison with 18S rDNA genes of other
plants in the public domain. A comparison of intraspecific
data information of nine 18S with that of 73 matK
and 86 rbcL sequences from GenBank revealed that
polymorphism, divergence and conservation is higher in
18S locus for Myristicaceae. Hence these markers may
be utilized for phylogenetic analysis, evaluation of
species richness during ecological surveys or for environmental
assessments. These molecular markers are
especially important due to the fact that the species
studied are mostly vulnerable and red-listed with limited
availability in endangered ecological niches.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/11791/1/Genet%20Resour%20Crop%20Evol%20%282014%29%2061523%E2%80%93535.pdf
Sheeja, T. E. and Rosana, O. B. and Swetha, V. P. and Shalini, R. S. and Siju, S. and Dhanya, R. (2014) The 18S rDNA gene discriminates between red-listed and unexplored ethnomedicinal species of Myristicaceae restricted to humid tropics of India. Genetic Resources and Crop Evolution, 61. pp. 523-535.