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Molecular identification and characterization of Fusarium spp. associated with sorghum seeds.

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Relation http://ir.cftri.com/11804/
http://dx.doi.org/10.1002/jsfa.6380
 
Title Molecular identification and characterization
of Fusarium spp. associated with sorghum
seeds.
 
Creator Divakara Shetty, T.
Parthasarathy, Santosh
Mohammed, Aiyaz
Mudili, Venkata Ramana
Hariprasad, P.
 
Subject 06 Sorghum
09 Food Microbiology
 
Description BACKGROUND: Fusarium spp. are not only pathogenic to plants but are also known as toxin producers that negatively affect
animal and human health. The identification of Fusarium spp. remains one of the most critical issues in fungal taxonomy. In
this study, different strains of Fusarium spp. were isolated from sorghum seed samples and identified at themolecular level by
tef-1α gene amplification. A multiplex polymerase chain reaction (mPCR) assay was developed to differentiate toxigenic and
non-toxigenic Fusarium spp. by designing a primer for the Fum21 gene along with the Fum1 and Fum8 genes. A competitive
direct enzyme-linked immunosorbent assay (CD-ELISA) was employed to assess the fumonisin-producing ability of Fusarium
spp. Phylogenetic analyses were performed using partial sequences of tef-1α and inter-simple sequence repeat (ISSR)markers
of different Fusarium spp.
RESULTS: All 27 isolates of Fusarium spp. were positive for the tef-1α gene and revealed the presence of F. verticillioides,
F. thapsina and F. cf. incarnatum-equiseti complex. The standardized mPCR assay distinguished toxigenic and non-toxigenic
F. verticillioides. Further, mPCR fumonisin-positive F. verticillioides isolates were also positive by CD-ELISA. The tef-1α gene
sequence was found to be useful in revealing intraspecific polymorphism to some extent. ISSR markers revealed a high level of
polymorphism among different isolates of Fusarium spp., and the dendrogram of ISSR analyses grouped the 27 isolates into
twomajor clusters.
CONCLUSION: The presentmethodprovided rapid andreliable detection of fumonisin-producing Fusarium spp. ThemPCRassay
couldbe analternative strategy to current conventional mycotoxin analytical techniques anda reliable tool forhigh-throughput
monitoring of major mycotoxin-producing fungi during the processing steps of food and feed commodities.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11804/1/J%20Sci%20Food%20Agric_2014_94_1132%E2%80%931139.pdf
Divakara Shetty, T. and Parthasarathy, Santosh and Mohammed, Aiyaz and Mudili, Venkata Ramana and Hariprasad, P. (2014) Molecular identification and characterization of Fusarium spp. associated with sorghum seeds. Journal of the Science of Food and Agriculture, 94. pp. 1132-1139.