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Identification and characterization of a basic thaumatin-like protein (TLP 2) as an allergen in sapodilla plum (Manilkara zapota).

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Relation http://ir.cftri.com/11827/
http:10.1002/mnfr.201300261
 
Title Identification and characterization of a basic
thaumatin-like protein (TLP 2) as an allergen in sapodilla
plum (Manilkara zapota).
 
Creator Venkatesh, L. Hegde
Hassan, G. Ashok Kumar
Sreenath, K.
Muralidhar Hegde, L.
Venkatesh, Y. P.
 
Subject 12 Allergy & Toxicology
24 Fruits
 
Description Scope: Cases of oral allergy syndrome following the ingestion of sapodilla plum (Manilkara
zapota) have been reported rarely. As the causative allergens are not known, the main objective
of this study was to identify and characterize the important allergens in sapodilla.
Methods and results: Allergy to sapodilla was diagnosed by case history, skin prick test, and
serum allergen–specific IgE. The allergen was detected by IgE immunoblotting, purified on
SP–Sepharose and characterized by native/SDS–PAGE, IEF, MS, and amino acid composition.
Several cases of allergy to sapodilla fruit were identified; majority of the sapodilla-allergic
subjects (6/7) experienced typical oral allergy syndrome symptoms, and allergen–specific IgE
to the purified protein was positive. The allergen has a pI ≥9.5 and high contents of arginine,
threonine, glycine, and cysteine. Circular dichroism revealed a secondary structure rich in beta
sheets/turns. Based on its N-terminal sequence of A-T-F-D-I-Q-N-N-C-X-Y-, the allergen (21
578 Da) was identified as a thaumatin-like protein by homology.
Conclusion: The causative allergen in sapodilla plum has been identified and characterized
as a highly basic thaumatin-like protein belonging to the pathogenesis-related protein (PR–5)
family, which has been recognized as a new family of conserved, cross-reactive plant allergens.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11827/1/Mol.%20Nutr.%20Food%20Res.2014_58_894-902.pdf
Venkatesh, L. Hegde and Hassan, G. Ashok Kumar and Sreenath, K. and Muralidhar Hegde, L. and Venkatesh, Y. P. (2014) Identification and characterization of a basic thaumatin-like protein (TLP 2) as an allergen in sapodilla plum (Manilkara zapota). Molecular Nutrition and Food Research, 58. pp. 894-902.