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Mass-Spectrometric Identification of T-Kininogen I/ Thiostatin as an Acute-Phase Inflammatory Protein Suppressed by Curcumin and Capsaicin.

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Relation http://ir.cftri.com/11835/
http://dx.doi.org/10.1371/journal.pone.0107565
 
Title Mass-Spectrometric Identification of T-Kininogen I/
Thiostatin as an Acute-Phase Inflammatory Protein
Suppressed by Curcumin and Capsaicin.
 
Creator Bina, Joe
Anitha, Nagaraju
Lalitha, R. Gowda
Venkatesha, Basrur
Lokesh, B. R.
 
Subject 09 Human Physiology
30 Spices/Condiments
 
Description Curcumin and capsaicin are dietary xenobiotics with well-documented anti-inflammatory properties. Previously, the
beneficial effect of these spice principles in lowering chronic inflammation was demonstrated using a rat experimental
model for arthritis. The extent of lowering of arthritic index by the spice principles was associated with a significant shift in
macrophage function favoring the reduction of pro-inflammatory molecules such as reactive oxygen species and
production and release of anti-inflammatory metabolites of arachidonic acid. Beyond the cellular effects on macrophage
function, oral administration of curcumin and capsaicin caused alterations in serum protein profiles of rats injected with
adjuvant to develop arthritis. Specifically, a 72 kDa acidic glycoprotein, GpA72, which was elevated in pre-arthritic rats, was
significantly lowered by feeding either curcumin or capsaicin to the rats. Employing the tandem mass spectrometric
approach for direct sequencing of peptides, here we report the identification of GpA72 as T-kininogen I also known as
Thiostatin. Since T-kininogen I is an early acute-phase protein, we additionally tested the efficiency of curcumin and
capsaicin to mediate the inflammatory response in an acute phase model. The results demonstrate that curcumin and
capsaicin lower the acute-phase inflammatory response, the molecular mechanism for which is, in part, mediated by
pathways associated with the lowering of T-kininogen I.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://ir.cftri.com/11835/1/PLOS%20ONE%20%20October%202014%20%20Volume%209%20%20Issue%2010.pdf
Bina, Joe and Anitha, Nagaraju and Lalitha, R. Gowda and Venkatesha, Basrur and Lokesh, B. R. (2014) Mass-Spectrometric Identification of T-Kininogen I/ Thiostatin as an Acute-Phase Inflammatory Protein Suppressed by Curcumin and Capsaicin. PLOS One, 9 (10). pp. 1-7.